{"id":2310,"date":"2023-08-24T11:18:03","date_gmt":"2023-08-24T11:18:03","guid":{"rendered":"https:\/\/weseq.it\/?post_type=ht_kb&#038;p=2310"},"modified":"2025-02-03T10:50:29","modified_gmt":"2025-02-03T09:50:29","slug":"transcriptome-sequencing","status":"publish","type":"ht_kb","link":"https:\/\/weseq.it\/en\/baza_wiedzy\/transcriptome-sequencing\/","title":{"rendered":"Transcriptome Sequencing"},"content":{"rendered":"<p>Celem poznania sekwencji transkryptomu oferujemy r\u00f3\u017cne podej\u015bcia w zale\u017cno\u015bci od stawianych hipotez badawczych:<\/p>\n\n\n\n<p>&#8211; <a href=\"https:\/\/weseq.it\/en\/baza_wiedzy\/rna-seq\/\" target=\"_blank\" rel=\"noreferrer noopener\">Total RNA<\/a> (biblioteki tworzone metod\u0105 rRNA depletion oraz zestawem dedykowanym dla small RNAseq)<br>&#8211; <a href=\"https:\/\/weseq.it\/en\/baza_wiedzy\/rna-seq\/#zastosowanie-rnaseq\" target=\"_blank\" rel=\"noreferrer noopener\">mRNA<\/a> (biblioteki tworzone metod\u0105 polyA lub stranded mRNA)<br>&#8211; <a href=\"https:\/\/weseq.it\/en\/baza_wiedzy\/small-rnaseq\/\" target=\"_blank\" rel=\"noreferrer noopener\">small RNA<\/a> (biblioteki tworzone zestawem dedykowanym do smallRNAseq)<br>&#8211; <a href=\"https:\/\/weseq.it\/en\/baza_wiedzy\/rna-seq\/#charakterystyka-techniczna-uslugi-rnaseq-deplecja-rrna\" target=\"_blank\" rel=\"noreferrer noopener\">lncRNA<\/a> (biblioteki tworzone metod\u0105 rRNA depletion)<br>&#8211; circRNA Sequencing (biblioteki tworzone metod\u0105 rRNA depletion)<br>&#8211; full-length transcript (Iso-Seq)<\/p>\n\n\n\n<p>&#8211; <a href=\"https:\/\/weseq.it\/en\/baza_wiedzy\/prokaryotic-mrnaseq\/\" target=\"_blank\" rel=\"noreferrer noopener\">Prokaryotic RNAseq<\/a> (biblioteki tworzone metod\u0105 rRNA depletion)<\/p>\n\n\n\n<p>Rodzaje rozwi\u0105za\u0144 celem tworzenia bibliotek:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>rRNA depletion<\/strong> &#8211; oparte jest o usuni\u0119cie rybosomalnego RNA w wyniku czego w materiale pozostaje zar\u00f3wno mRNA, lncRNA, circRNA jak i smallRNA. Rozwi\u0105zanie to dedykowane jest r\u00f3wnie\u017c do bada\u0144 organizm\u00f3w prokariotycznych kt\u00f3rych transkrypty nie zawieraj\u0105 ogon\u00f3w polyA.<\/li>\n\n\n\n<li><strong>polyA<\/strong> <strong>enrichment<\/strong>&#8211; oparte o wy\u0142apywanie z materia\u0142u genetycznego tylko tych transkrypt\u00f3w kt\u00f3re zawieraj\u0105 ogony polyA, czyli transkrypt\u00f3w mRNA. Podej\u015bcie zalecane gdy celem projektu jest poznanie r\u00f3\u017cnic w ekspresji gen\u00f3w badanego organizmu. <\/li>\n\n\n\n<li><strong>stranded mRNA<\/strong> &#8211; pozwala na precyzyjne okre\u015blenie orientacji nici mRNA w celu wykrycia transkrypt\u00f3w antysensownych, alternatywnych transkrypt\u00f3w, fuzji gen\u00f3w oraz ekspresja specyficznych dla konkretnych alleli.<\/li>\n<\/ul>\n\n\n\n<p>Wszystkie informacje dotycz\u0105ce RNAseq \u0142\u0105cznie z chcarakterystyk\u0105, przebiegiem procesu oraz wymaganiami zosta\u0142y opisane w karcie produktu <a href=\"https:\/\/weseq.it\/en\/baza_wiedzy\/rna-seq\/\" target=\"_blank\" rel=\"noreferrer noopener\">tutaj<\/a>. <\/p>\n\n\n\n<p>Celem poznania cen oferowanych us\u0142ug zalecamy wype\u0142nienie <a href=\"https:\/\/weseq.it\/en\/specyfikacja-projektu\/\">formularza specyfikacji projektu<\/a> i\/lub zapoznanie si\u0119 z artyku\u0142em dotycz\u0105cym <a href=\"https:\/\/weseq.it\/en\/centrum_wiedzy\/wycena-uslug-sekwencjonowania\/\">wyceny us\u0142ug<\/a>.<\/p>","protected":false},"excerpt":{"rendered":"<p>Celem poznania sekwencji transkryptomu oferujemy r\u00f3\u017cne podej\u015bcia w zale\u017cno\u015bci od stawianych hipotez badawczych: &#8211; Total RNA (biblioteki tworzone metod\u0105 rRNA depletion oraz zestawem dedykowanym dla small RNAseq)&#8211; mRNA (biblioteki tworzone metod\u0105 polyA lub stranded mRNA)&#8211; small RNA (biblioteki tworzone zestawem dedykowanym do smallRNAseq)&#8211; lncRNA (biblioteki tworzone metod\u0105 rRNA depletion)&#8211; circRNA&#8230;<\/p>","protected":false},"author":1,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"ht-kb-category":[90],"ht-kb-tag":[81,78,82,83,79,80,77],"class_list":["post-2310","ht_kb","type-ht_kb","status-publish","format-standard","hentry","ht_kb_category-lista-uslug-ngs","ht_kb_tag-lncrnaseq","ht_kb_tag-mrnaseq","ht_kb_tag-polya","ht_kb_tag-rrna-depletion","ht_kb_tag-smallrnaseq","ht_kb_tag-srnaseq","ht_kb_tag-transcriptome"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v24.9 (Yoast SEO v27.4) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Transcriptome Sequencing - weSEQ.IT<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/weseq.it\/en\/baza_wiedzy\/transcriptome-sequencing\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Transcriptome Sequencing\" \/>\n<meta property=\"og:description\" content=\"Celem poznania sekwencji transkryptomu oferujemy r\u00f3\u017cne podej\u015bcia w zale\u017cno\u015bci od stawianych hipotez badawczych: &#8211; 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