{"id":2841,"date":"2024-04-02T12:55:23","date_gmt":"2024-04-02T11:55:23","guid":{"rendered":"https:\/\/weseq.it\/?post_type=ht_kb&p=2841"},"modified":"2024-04-02T12:55:27","modified_gmt":"2024-04-02T11:55:27","slug":"pellet-z-kultur-bakteryjnych","status":"publish","type":"ht_kb","link":"https:\/\/weseq.it\/en\/baza_wiedzy\/pellet-z-kultur-bakteryjnych\/","title":{"rendered":"Pellet z kultur bakteryjnych"},"content":{"rendered":"

Przygotowanie pr\u00f3bek kultur bakteryjnych jest kluczowym etapem maj\u0105cym na celu zabezpieczenie cennego materia\u0142u do izolacji kwas\u00f3w nukleinowych na bazie kt\u00f3rych zostan\u0105 nast\u0119pnie przygotowane biblioteki do sekwencjonowania i wykonane sekwencjonowanie.<\/p>\n\n\n\n

Aby przygotowa\u0107 pr\u00f3bki do wysy\u0142ki nale\u017cy post\u0119powa\u0107 wed\u0142ug poni\u017cej instrukcji:<\/strong>
1. Wyhoduj bakterie na po\u017cywce p\u0142ynnej do po\u0142owy fazy logarytmicznej lub wczesnej fazy stacjonarnej.
2. Zbierz co najmniej 4 * 10\u2079 kom\u00f3rek (lub 10 ml hodowli, je\u015bli analiza liczby kom\u00f3rek nie jest mo\u017cliwa).
3. Uzyskaj pellet przez odwirowanie i odrzucenie supernatantu.
4. Zamro\u017a pellet w temperaturze < -18\u00b0C.
5. Pellet nale\u017cy wys\u0142a\u0107 na suchym lodzie.<\/p>\n\n\n\n

Tak przygotowany i wys\u0142any pellet zapewnia odpowiedni materia\u0142 do przeprowadzenia izolacji kwas\u00f3w nukleinowych.<\/p>","protected":false},"excerpt":{"rendered":"

Przygotowanie pr\u00f3bek kultur bakteryjnych jest kluczowym etapem maj\u0105cym na celu zabezpieczenie cennego materia\u0142u do izolacji kwas\u00f3w nukleinowych na bazie kt\u00f3rych zostan\u0105 nast\u0119pnie przygotowane biblioteki do sekwencjonowania i wykonane sekwencjonowanie. Aby przygotowa\u0107 pr\u00f3bki do wysy\u0142ki nale\u017cy post\u0119powa\u0107 wed\u0142ug poni\u017cej instrukcji:1. Wyhoduj bakterie na po\u017cywce p\u0142ynnej do po\u0142owy fazy logarytmicznej lub wczesnej…<\/p>","protected":false},"author":1,"comment_status":"open","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"ht-kb-category":[40],"ht-kb-tag":[160,161,157],"class_list":["post-2841","ht_kb","type-ht_kb","status-publish","format-standard","hentry","ht_kb_category-przygotowanie-przesylki-i-logistyka-odbioru","ht_kb_tag-bakterie","ht_kb_tag-kultura","ht_kb_tag-pellet"],"yoast_head":"\nPellet z kultur bakteryjnych - weSEQ.IT<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/weseq.it\/en\/baza_wiedzy\/pellet-z-kultur-bakteryjnych\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Pellet z kultur bakteryjnych\" \/>\n<meta property=\"og:description\" content=\"Przygotowanie pr\u00f3bek kultur bakteryjnych jest kluczowym etapem maj\u0105cym na celu zabezpieczenie cennego materia\u0142u do izolacji kwas\u00f3w nukleinowych na bazie kt\u00f3rych zostan\u0105 nast\u0119pnie przygotowane biblioteki do sekwencjonowania i wykonane sekwencjonowanie. 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