Knowledge Base/NGS services/Sequencing Methods

SEQUENCING METHODS

Sequencing Methods

All the methods we offer, organised by material and research question. If you are not sure which one to choose, start with the comparisons above.

COMPARISONS METHOD SELECTION Before you choose a method Answers when you are choosing between two options. 1 comparison DNA GENOMICS Genomics Genome sequencing and analysis. 3 services RNA TRANSCRIPTOMICS Transcriptomics Analysis of gene expression, transcripts and their isoforms. 5 services CHROMATIN EPIGENOMICS & 3D GENOME Epigenomics and 3D genome organisation Analysis of genome regulation, chromatin accessibility and its spatial organisation. 3 services COMPLEX SAMPLE MICROBIOME & METAGENOMICS Microbiome and metagenomics Characterisation of microorganisms and their functional potential in complex samples. 2 services SINGLE CELLS SINGLE-CELL & SPATIAL OMICS Single cells and spatial analysis Analysis of cell and nucleus heterogeneity and the spatial organisation of gene expression. 3 services LIBRARIES PRE-MADE LIBRARY SEQUENCING Sequencing of pre-made libraries For researchers who prepare their own NGS libraries. 1 service

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COMPARISONS

METHOD SELECTION

Before you choose a method

Metatranscriptomics or prokaryotic RNA-seq: which should you choose? How the two technologies differ and which one to select for your research objective.

Amplicon sequencing or shotgun metagenomics: which method should you choose?

Compare amplicon sequencing and shotgun metagenomics: explore the scope of services, applications, similarities and differences to make an informed choice of method for your research objectives.

DNA

GENOMICS

Genome sequencing and analysis

Whole Genome Sequencing WGS whole-genome sequencing

Whole Genome Sequencing is a comprehensive sequencing method for determining an organism's complete genome sequence.

Human WGSPlant and Animal WGSBacterial and Fungal WGS

Whole Exome Sequencing WES whole-exome sequencing

Whole Exome Sequencing enables analysis of protein-coding regions, including exons and adjacent sequences.

Genotyping & Reduced Representation Sequencing genotyping and reduced-representation genome sequencing

Specific-Locus Amplified Fragment Sequencing SLAF-seq

RNA

TRANSCRIPTOMICS

Analysis of gene and transcript expression, including transcript isoforms

Messenger RNA Sequencing mRNA-seq mRNA sequencing

Poly(A)-tailed mRNA fraction. Choose this method when your primary objective is gene-expression profiling.

Total RNA Sequencing total RNA-seq rRNA-depleted RNA sequencing

Following ribosomal RNA depletion, the sequencing data contain mRNA, lncRNA and circRNA reads.

Small RNA Sequencing small RNA-seq small RNA sequencing

Full-Length Transcriptome Sequencing full-length transcripts

Full-length transcript sequencing enables discrimination between isoforms originating from the same gene.

Prokaryotic RNA Sequencing RNA-seq prokaryotic transcriptomics

Gene-expression profiling in bacteria, with rRNA depletion tailored to prokaryotic samples.

CHROMATIN

EPIGENOMICS & 3D GENOME

Analysis of genome regulation, chromatin accessibility and 3D genome organisation

Whole-Genome Bisulfite Sequencing WGBS DNA methylation

Genome-wide DNA methylation profiling at single-base resolution.

Assay for Transposase-Accessible Chromatin with Sequencing ATAC-seq chromatin accessibility

Mapping open chromatin regions where the genome is accessible to transcriptional regulators.

High-Throughput Chromosome Conformation Capture Hi-C 3D genome organisation

Analysis of spatial contacts within the genome to identify DNA regions located in close proximity in the cell nucleus.

COMPLEX SAMPLE

MICROBIOME & METAGENOMICS

Characterisation of microorganisms and their functional potential in complex samples

Amplicon Sequencing amplicon sequencing

Targeted 16S rRNA Gene and Internal Transcribed Spacer (ITS) Amplicon SequencingFull-Length 16S rRNA Gene and ITS Amplicon Sequencing

Shotgun Metagenomic Sequencing shotgun metagenomics

SINGLE CELLS

SINGLE-CELL & SPATIAL OMICS

Profiling cellular and nuclear heterogeneity and the spatial organisation of gene expression

10x Genomics Single-Cell RNA Sequencing Single Cell and Spatial Omics single-cell gene expression

10x Genomics Single-Cell ATAC Sequencing scATAC-seq single-cell chromatin accessibility

Spatial Transcriptomics ST spatial transcriptomics

LIBRARIES

PRE-MADE LIBRARY SEQUENCING

Sequencing of pre-made libraries

For researchers who prepare NGS libraries themselves and need sequencing only.

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