Sequencing Methods

Amplicon sequencing or shotgun metagenomics: which method should you choose?

Compare amplicon sequencing and shotgun metagenomics: explore the scope of services, applications, similarities and differences to make an informed choice of method for your research objectives.

mRNA-seq or Total RNA-seq? Which method should you choose?

Learn about the key differences between mRNA-seq with Poly(A) selection and Total RNA-seq with rRNA depletion, their applications and the range of transcripts analysed, so you can choose the library preparation method best suited to your study objective and biological material type.

Illumina, PacBio or Oxford Nanopore? Which technology should you choose?

Learn about the key differences between Illumina, PacBio HiFi and Oxford Nanopore sequencing technologies, their applications and analytical capabilities, so you can choose the platform or hybrid approach best suited to your study objective, quality requirements and project budget.

De novo genome assembly - Illumina, PacBio, Nanopore or a hybrid approach?

Find out which sequencing technology to choose for de novo genome assembly - Illumina, PacBio HiFi, Oxford Nanopore or a hybrid approach - and how it affects the quality, completeness and cost of obtaining a genome sequence.

WGS, WES or a gene panel? Which method should you choose?

Learn about the key differences between WGS, WES and gene panel sequencing, their applications and their ability to detect genetic variants, so you can choose the method best suited to your study objective, required sensitivity and project budget.

Metatranscriptomics for studying microbiome activity

Explore the key differences between metagenomics and metatranscriptomics, their applications and analytical capabilities to choose the sequencing method best suited to your study objective.

Whole Genome Sequencing WGS whole-genome sequencing

Whole Genome Sequencing is a comprehensive sequencing method for determining an organism's complete genome sequence.

Human WGSPlant and Animal WGSBacterial and Fungal WGS

Whole Exome Sequencing WES whole-exome sequencing

Whole Exome Sequencing enables analysis of protein-coding regions, including exons and adjacent sequences.

Genotyping & Reduced Representation Sequencing genotyping and reduced-representation genome sequencing

Specific-Locus Amplified Fragment Sequencing SLAF-seq

Messenger RNA Sequencing mRNA-seq mRNA sequencing

Poly(A)-tailed mRNA fraction. Choose this method when your primary objective is gene-expression profiling.

Total RNA Sequencing total RNA-seq rRNA-depleted RNA sequencing

Following ribosomal RNA depletion, the sequencing data contain mRNA, lncRNA and circRNA reads.

Small RNA Sequencing small RNA-seq small RNA sequencing

Full-Length Transcriptome Sequencing full-length transcripts

Full-length transcript sequencing enables discrimination between isoforms originating from the same gene.

Prokaryotic RNA Sequencing RNA-seq prokaryotic transcriptomics

Gene-expression profiling in bacteria, with rRNA depletion tailored to prokaryotic samples.

Whole-Genome Bisulfite Sequencing WGBS DNA methylation

Genome-wide DNA methylation profiling at single-base resolution.

Assay for Transposase-Accessible Chromatin with Sequencing ATAC-seq chromatin accessibility

Mapping open chromatin regions where the genome is accessible to transcriptional regulators.

High-Throughput Chromosome Conformation Capture Hi-C 3D genome organisation

Analysis of spatial contacts within the genome to identify DNA regions located in close proximity in the cell nucleus.

ChIP-seq

Find out how to identify protein-binding sites on DNA and analyse histone modifications, and which samples, sequencing parameters and analyses the service covers.

Amplicon Sequencing amplicon sequencing

Targeted 16S rRNA Gene and Internal Transcribed Spacer (ITS) Amplicon SequencingFull-Length 16S rRNA Gene and ITS Amplicon Sequencing

Shotgun Metagenomic Sequencing shotgun metagenomics

Metatranscriptomic Seq

Find out how to analyse gene expression in microbial communities and which sample types, sequencing parameters and analyses are covered by the service.

10x Genomics Single-Cell RNA Sequencing scRNA-seq single-cell gene expression

10x Genomics Single-Cell ATAC Sequencing scATAC-seq single-cell chromatin accessibility

Spatial Transcriptomics ST spatial transcriptomics

Sequencing of pre-made libraries

For researchers who prepare NGS libraries themselves and need sequencing only.