Because the quality of the input material used for library preparation is a key factor affecting sequencing results, we offer nucleic acid extraction from the supplied material. DNA or RNA extraction can be added to any weSEQ.IT service that includes library preparation and sequencing.
What the amount of material depends on
The minimum amount of material required for extraction depends on several key factors, including:
- Material Typemeaning the material / explant from which nucleic acids will be extracted. We also recommend considering how rich the tissue / material is in cell nuclei.
- Sample FormatThe format of the submitted material is also important, including whether it is a liquid, such as blood, plasma or buffer containing sorted cells, and whether the material will be frozen.
- Sequencing PlatformThe amount of DNA/RNA required for library preparation depends on the sequencing platform, which also determines the minimum amount of material required for DNA/RNA extraction.
- Analysis ObjectiveThe objective and type of the planned analysis determine the required amount of material because of the specific minimum DNA / RNA input requirements.
How to order isolation
To receive a personalised quote including DNA or RNA extraction, please complete the project specification form available on our website at https://weseq.it/specyfikacja-projektu/. In the form, indicate that nucleic acid extraction is required and provide all necessary information based on the guidance above. Our experienced team will tailor the extraction process to each client's individual requirements while maintaining the highest service standards.
Selected projects and client publications
See which institutions we perform next-generation sequencing (NGS) for and explore scientific publications that draw on the data we have supplied.