PROJECT SPECIFICATION

Please fill in the form below regarding the planned analysis specification to best match the NGS service offer to your project. We recommend that you complete all fields appropriately.
If you have any questions or concerns, please contact the Customer Support Department: support@weSEQ.IT

By completing the project specification form:

your needs and requirements will be precisely specified,
we will avoid communication errors,
the inquiry will be immediately sent to the valuation,
an additional discount will be added for self-completion of the inquiry.

Project specification form

"*" indicates required fields

Step 1 of 4

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Section 1 – General Information

ID:

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Data wpłynięcia zapytania:

YYYY slash MM slash DD

The following data will be included in the official offer.

Imię i Nazwisko*

Name Surname

Email*

Phone*

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Nazwa firmy / Instytutu-nieaktywne*

Company/Institute name*

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VAT number*

Please enter a number from 0000000000 to 9999999999.

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Nazwa projektu – zostanie przedstawiona na oficjalnej ofercie.*

Planned date of samples shipment for analysis:*

YYYY dash MM dash DD

Purpose of inquiry:*

Service valuation before orderingZaznacz tę opcję, jeśli potrzebujesz wyceny przed zleceniem zamówienia. Klienci, którzy mają aktualne i realne potrzeby są traktowani priorytetowo.

Service valuation for the grant proposalZaznacz tę opcję, jeśli potrzebujesz wyceny niezbędnej do ogłoszenia przetargu lub złożenia wniosku o finansowanie projektu. Przedstawimy ofertę wstępną wraz z informacjami jakich zmian można się spodziewać.

The offer is needed only to fulfill the formalities of 3 offers in a public tender.Zaznacz tę opcję, jeśli nie możesz skorzystać z naszych usług, gdyż firma do zrealizowania zamówienia została już wybrana. Innymi słowy, gdy potrzebujesz jedynie podkładki niezbędnej do spełniania wymogu administracyjnego. Przedstawimy ofertę, która będzie spełniała wymogi trzeciej oferty.

Market analysisZaznacz tę opcję, jeśli chcesz z ciekawości/poglądowo dowiedzieć się, jaką posiadamy ofertę i poznać ceny, ale nie zamierzasz w najbliższym czasie skorzystać z naszych usług. Przedstawimy w mailu ofertę wstępną.

When are the results of the research grant application competition scheduled to be announced?*

YYYY dash MM dash DD

Why are you conducting market analysis?*

The more we know about your needs, the more accurate information we can provide.

What is the purpose of the planned analysis?*

Understanding your precise goal is a key step in the offer creation process. By knowing your goal, we will be able to better interpret your needs and propose a variety of available options tailored to your requirements.

Consent to the processing of personal data*

I consent to process my personal data contained in the project specification form for the purposes necessary to answer my inquiry.

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Section 2 – Type of service

Service type:*

Packages: Genomics, Transcriptomics or Epigenomics (per sample)Pakiety zawierają usługę przygotowania bibliotek oraz sekwencjonowania w wybranej konfiguracji.

NGS sequencing of customer-prepared libraries (full or partial line/cell)

Sanger sequencing

Packages: Genomics, Transcriptomics or Epigenomics (per sample):

Project type:*

NGS analysis of microorganisms

Transcriptomics of eukaryotic organisms

Plant and Animal Genomics

Human Genomics

Epigenomics

Other

Microbial analysis type:*

Amplicon 16S/18S/ITS

Metagenomics

Whole Bacterial Genome Sequencing

Whole Fungal Genome

16S/18S/ITS/ Platform:*

Illumina,

ONT,

Pac-Bio.

Choosing ONT or PacBio means choosing sequencing of the entire V1-V9 region of 16S or entire ITS.

Illumina (Amplicon 16S/18S/ITS/ Ilumina):*

V3 + V4,

V4,

V4 + V5,

V5 + V7,

ITS1 (for fungi analysis),

ITS2 (for fungi analysis),

Other region

(you can choose two: one for bacteria analysis and one for fungi analysis from one sample)

Region:*

(Proszę wpisać rodzaj regionu)

Choose the number of readings/tags per sample:*Mowa o totalnej ilości odczytów na próbkę tzn. 100k tags = 100k odczytów w sumie (50k odczytów sparowanych).

50K

100K

200K

Other

Number of reads (tags) per sample (Pac-Bio/ONT):*

10K

20K

30K

100K

Other

Choose the Metagenome analysis platform:*

Illumina

ONT

Pac-Bio

Select the amount of metagenomic sequencing data you need per one sample (Illumina):*

3 Gb

6 Gb

9 Gb

10 Gb

20 Gb

Other

Select the amount of metagenomic sequencing data you need per one sample (ONT/PacBio):*

6 Gb

10 Gb

Other

Whole Bacterial Genome Sequencing:*

200x, 5 Mbp genome (de novo) – Illumina

100x, >8 Mbp (resequencing) – Illumina

30x HIFI, >8 Mbp genome (resequencing) – Pac-Bio

100x ONT, >8 Mbp genome (resequencing) – Nanopore

I need a different configuration.

Whole Fungal Genome Sequencing:*

50x, <60 Mbp – survey; Illumina

100x, <60 Mbp – draft; Illumina

100x, <60 Mbp – Hi-C based

30x HiFi, <60 Mbp Fine Genome – Pac-Bio

100x, <60 Mbp – Fine Genome- Nanopore

I need a different configuration.

Select the sequencing platform for WGS:*

Illumina

Pac-Bio

ONT

Other

Needed genome coverage:*

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Typ pakietu:

Transcriptomics of eukaryotic organisms:*

Eucaryotic mRNA Sequencing (polyA enrichment)

lncRNA Sequencing: lncRNAseq + mRNAseq (rRNA depletion)

small RNA Sequencing (rRNA depletion)

circRNA Sequencing (rRNA depletion):

Total RNA Sequencing: smallRNAseq + mRNAseq + lncRNAseq (rRNA depletion)

Full length mRNA Sequencing (Pac-Bio / Nanopore)

Select the required amount of mRNAseq data per sample (polyA):*

3 Gb (20M reads)

4,5 Gb (30M reads)

6 Gb (40M reads)

7,5 Gb (50M reads)

9 Gb (60M reads)

10 Gb (66M reads)

12 Gb (80M reads)

15 Gb (100M reads)

Other

Choose the library type for mRNAseq analysis:*

cDNA

Straded / Directional

Select the required amount of lncRNAseq data per sample:*

10 Gb

12 Gb

15 Gb

16 Gb

Other

Select the required amount of small RNAseq data per sample:*

10 M SE reads

20 M SE reads

Other

Select the required amount of circ RNAseq data per sample:*

16 Gb (minimum amount of data per sample to detect circRNAs)

Other

Wybierz wymaganą ilość danych z Total RNAseq (rRNA depleted)?*

4,5 Gb (30M reads)

6 Gb (40M reads)

7,5 Gb (50M reads)

9 Gb (60M reads)

10 Gb (66M reads)

12 Gb (80M reads)

15 Gb (100M reads)

30 Gb (200M reads)

Other

Wybierz wymaganą ilość danych z full length mRNA-seq (Pac-Bio/ONT):*

4 Gb

6 Gb

12 Gb

20 Gb

40 Gb

Other

Wybierz platformę do analizy full lenght mRNAseq:*

Pac-Bio

ONT

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Genomika Roślin i Zwierząt:

Select the project type :*

WGS (Whole Genome Seq)

SLAF-seq (Specific locus amplified fragment sequencing – optimised ddRADseq procedure.

Select the number of reads per sample from SLAF-seq*

100K tags

200K tags

300K tags

400K tags

Other

Enter the expected amount of data in GB from sequencing of each sample [GB]:*

Choose the sequencing platform for WGS:*

Illumina

Pac-Bio

ONT

Other

Is GWAS (Genome-Wide Association Studies) analysis required?*

Yes

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Human Genomics:

Human Genomics:*

Human WES (Whole Exome Seq)

Human WGS (Whole Genome Seq)

Select the amount of data and exome coverage for the Human WES project per sample:*

50x, 5Gb

100x, 10Gb

12Gb Germline Variant Detection

15Gb Somatic Variant Detection

Other

Choose the platform for WGS:*

Illumina

ONT

Pac-Bio

BGI

Determine the amount of data and genome coverage per sample:*

90 Gb, 30x – Germline Variant Detection

240Gb, 80x – Somatic Variant Detection

Other

Is it necessary to prepare libraries with barcodes?*

Yes

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Epigenomics project type:

Epigenomics project type:*

WGBS

Hi-C

ATAC

WGBS (Epigenomics):

Select the organism for the WGBS analysis:*

Human / Rat / Mouse

Plant / Animal

Specify how much data per sample and what genome coverage is needed:*

30x, 90Gb

30x, 90Gb (low input)

Other

Specify how much data per sample [Gb] and what genome coverage is needed:*

Low input:*Termin ‘low input’ odnosi się do przypadków, w których konieczne jest zastosowanie specjalnego podejścia do materiału o bardzo niskiej ilości lub koncentracji.

Yes

Not applicable

Hi-C (Epigenomics):

Enter how much data per sample is needed [GB]*

ATAC (Epigenomics):

How much data per sample and what genome coverage is needed [M of reads]?*

Other service (project type):

Describe in detail the type of service you need:*

Select your preferred sequencing platform:*

Illumina

Pac-Bio

ONT

Other

Enter how much data you need per sample [in GB or M of reads]:*

NGS sequencing of customer-prepared libraries (full or partial line/cell)

Provide the name of the kit that will be used to prepare libraries for sequencing:*

What material will be used to prepare libraries for sequencing?*

Genomic DNA

ChIP DNA

RNA

Amplicon

Single cells / nuclei

Bulk ATAC-seq

Other

Number of libraries for analysis:*

Enter the number of libraries for sequencing (e.g. if 3 pools (3 tubes) are sent and there are 100 libraries in each pool, the total libraries number is 300.

Required output per library (per 1 library) [M]*Na podstawie tej informacji określana jest głębokość sekwencjonowania, inaczej mówiąc ilość danych jakie otrzymasz po sekwencjonowaniu na jedną próbkę.

Enter the value in millions of reads in total (not pairs of reads )

The estimated library size is:*Podaj długość biblioteki (w parach zasad), która zostanie do nas wysłana do sekwencjonowania.

Choose the preferred sequencing mode:*

1x50bp

2x150bp

2x250bp

2x300bp

Preferred sequencing platform:*

Illumina

Other

Name of preferred sequencing platform:*

Do the samples contain standard Illumina sequencing primer binding sites?*

Yes for both reads: Read 1 and Read 2

Yes, for one read only

Do the samples contain standard Illumina indexing primer binding sites?*

Yes, they contain single i7 or equivalent indexing sequences;

Yes, they contain dual indexing sequences i5 and i7 or their equivalents;

Yes, but we did not add any indexing sequences;

How long are the indexes?*

6bp (most single index),

8bp (most dual index),

10bp,

16bp,

24bp,

Other

Do you need PhiX spike-in?*

Yes

Indicate the degree of diversity of libraries:*

High level of diversity,

Low level of diversity.

Sanger sequencing

Samples will be sent:*

in Eppendorf tubes,

on 96-well plates.

Will the samples be mixed with the starter (so-called premix)?*

Yes, samples will be mixed.

No, the starter will be delivered in a separate tube.

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Wspólne pytania:

Follow-up questions:

Additional information:

The more detailed information you can provide, the more effectively we will adapt our offer to your needs. We treat each inquiry individually, to find the perfect solution that meets your expectations.

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Czy potrzebujesz pomocy technicznej?

Do you need technical support with this query?*

Yes

Tell us, how can we help you?

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Sekcja 3 – dodatkowe informacje:

Should the service include the isolation of nucleic acids from the provided samples?*

Yes

What material will be sent for isolation?:*

(e.g. tissues/cells/pellets/FFPE/blood etc.)

Should the service include bioinformatics analysis?*

Yes

Expectations regarding bioinformatics analysis:

What should the analysis include? State your expectations or examples for the analysis.

Enter the number of samples to be analyzed:*

Please enter a number greater than or equal to 1.

In this field, enter the number of individual tubes that will be sent to the laboratory. Each tube may contain a single sample, a library or a pool of libraries.

Will all samples be shipped in one shipment?*

Yes, samples will be sent all at once.

No, samples will be sent in batches.

Enter the number of batches:*

Please enter a number from 1 to 500.

The cost of sample transport and data delivery will be added to each batch.

Enter the analysed species:*

Latin name required.

Enter the genome size of the analyzed organism:*

Will the samples contain infectious or viral material?*

Yes

What type of infectious or viral material might be present in the samples?*

Select preferred method of data delivery after the service:*

Private link with access to data in an encrypted folder online.

External drive (recommended for all projects which final data volume exceeds 500Gb.)

The project purpose:*

Research

Clinical

Will the service be ordered on the basis of public procurement?*

Yes, but details have not been published yet

Yes, the inquiry has already been published.

Public inquiry/tender details:

Website address with the published query:

Inquiry / Tender details:

Max. file size: 2 GB.

The inquiry is valid until:

YYYY dash MM dash DD

Were there any doubts while filling out the form that require technical support?*

Yes

Opisz wątpliwości wymagające wsparcia technicznego:*

Additional information:

Payment:

By choosing 100% prepayment on an advance invoice, you will receive the best offer. You can find more about this on our website: https://weseq.it/baza_wiedzy/metody-platnosci/

Select your preferred payment method:*

PrepaymentZyskaj dostęp do naszej najkorzystniejszej oferty cenowej, dokonując przedpłaty.

Payment after the service is completed

We offer three payment options based on prepayment principles:*

On the basis of an advance invoice (standard)Faktura zaliczkowa zostanie wystawiona po otrzymaniu płatności. Faktura końcowa zostanie wystawiona po wykonaniu usługi.

Invoice issued after receiving the samples.Ta opcja jest dla klientów, którzy potrzebują pilnie faktury.

Payment by voucher / prepaid card

You can find more about it in our knowledge base: https://weseq.it/baza_wiedzy/metody-platnosci/

Provide the voucher:*

Max. file size: 2 GB.

Podaj kod bonu:*

Do you have a voucher?*

Yes

Enter the voucher code:*

How did you hear about weSEQ.IT?*

from an internet search engine;

from social media;

recommendation;

from a conference/training;

from e-mail;

from the account manager;

I am already a weSEQ.IT customer;

Other

Who recommended us?*

Thanks to this information, we will be able to thank the person who recommended us.

Rate the readability and ease of use of the form:*

6 - excellent

5 - very good

4 - good

3 - satisfactory

2 - marginal

1 - unsatisfactory

Tell us what we can improve:

We appreciate your opinions and comments. We want to develop and improve our services. That's why we really care about your feedback.