The Pre-made Library Sequencing service is intended for researchers who perform NGS library preparation in-house and only require high-quality sequencing on our platforms.
1. Technical requirements for samples (libraries)
To ensure the highest data quality and optimal process efficiency, libraries must be supplied in the appropriate amount and concentration. The required library concentration and volume depend on the planned service and the total data output per library (Gb). Please provide samples according to the following specification:
| Planned data output | Minimum library concentration | Minimum volume |
|---|---|---|
| Gb ≤ 10 | ≥ 1 nM | ≥ 25 µL |
| 10 < Gb ≤ 50 | ≥ 2 nM | ≥ 25 µL |
| 50 < Gb ≤ 100 | ≥ 3 nM | ≥ 25 µL |
| Gb > 100 | ≥ 4 nM | ≥ 25 µL |
2. Required information (Information Form)
Before a quote can be prepared and the feasibility of sequencing pre-made libraries assessed, detailed information about the library preparation procedure must be provided, including:
Selected projects and client publications
See which institutions we perform next-generation sequencing (NGS) for and explore scientific publications that draw on the data we have supplied.