To prepare cell samples correctly for RNA extraction, please follow the guidelines below.
Recommended number of cells per sample: 1 × 10⁷ cells
1. Adherent cells
01Wash adherent cells with PBS (5 mL, RNase-free).
02Remove PBS and add Trizol buffer.
03Use a pipette to collect the cell suspension and disperse any visible clumps, mixing until the suspension becomes clear.
04Transfer the suspension to 2 mL RNase-free centrifuge tubes.
05Store the tubes at -80 °C.
2. Suspension cells
01Wash suspension cells with PBS (1 mL, RNase-free).
02Transfer the suspension to 2 mL RNase-free centrifuge tubes.
03Pellet the cells by centrifugation at 3000 g.
04Add Trizol buffer and use a pipette to collect the suspension and disperse any visible clumps, mixing until the suspension becomes clear.
05Store the tubes at -80 °C.
3. Other cases
- For users without access to Trizol buffer, cells can be flash-frozen in liquid nitrogen after washing and then stored at -80 °C.
Shipment
- Samples should be packed in dry ice to ensure material stability and quality during transport.
- Sample preparation details are provided here.
RELATED ARTICLES
DNA or RNA extractionWhich buffer should you use to transport FACS-sorted cells for RNA analysis?Recommendations for sample preparation before shipmentSample typesSample requirements - DNA/RNASample requirements - TISSUESKEYWORDS
RNA isolationRNA from cellsadherent cellssuspension cellsTrizolPBSRNA extractioncell culture
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