Transcriptomic studies make it possible to determine which genes are active in the biological material being analysed and how their expression levels vary between samples, experimental conditions or developmental stages. Depending on the project objectives, the analysis may cover coding RNA, long and short non-coding RNAs, circular RNAs, full-length transcripts and their alternative isoforms. We offer solutions for analysing the transcriptomes of eukaryotic and prokaryotic organisms. We help select the library preparation method, sequencing technology, required amount of data and scope of bioinformatics analysis according to the sample type, organism and research objective.
Choose the service you are interested in to learn about Sample Requirements - DNA/RNA, available sequencing options and the possible scope of data analysis.
- Total RNA (libraries prepared using rRNA depletion and a kit dedicated to small RNA-seq)
- mRNA (libraries prepared using poly(A) selection or stranded mRNA)
- small RNA (libraries prepared using a kit dedicated to small RNA-seq)
- lncRNA (libraries prepared using rRNA depletion)
- full-length transcript (long-read sequencing)
- Prokaryotic RNA-seq (libraries prepared using rRNA depletion)
Which service should you choose?
Do you primarily want to analyse protein-coding genes?
Choose mRNA-seq. This method enables quantitative gene expression analysis and comparison of transcriptional profiles across samples.
In addition to mRNA, do you also want to analyse lncRNA or circRNA?
Consider total RNA sequencing with rRNA depletion, which covers a broader range of RNA classes than standard mRNA-seq.
Are you interested in miRNAs and other small RNAs?
Choose Small RNA Sequencing, designed for the analysis of short regulatory RNA molecules.
Do you want to distinguish full-length transcript isoforms?
Choose full-length transcriptome sequencing. Full-length transcript reads enable more accurate analysis of alternative splicing and isoform structure.
Are you planning to analyse gene expression in bacteria?
Choose prokaryotic RNA sequencing with rRNA depletion tailored to prokaryotic material.
Selecting the appropriate method depends on the type of RNA being analysed, the organism, sample quality and the research question.
Provide us with information about the samples, the number of experimental groups, the expected scope of analysis and the project objective, and we will help you select a suitable workflow.
COMPLETE THE PROJECT SPECIFICATION → here
Not sure which method would be best? Fill in the available information and we will be happy to help you plan the experiment.
BOOK A CONSULTATION → here