Preparing bacterial culture samples is a critical step in preserving valuable material for nucleic acid extraction, which will subsequently be used for library preparation and sequencing.
Follow the instructions below to prepare samples for shipment:
01Grow the bacteria in liquid medium to the mid-log phase or early stationary phase.
02Collect at least 4 * 10⁹ cells (or 10 ml of culture if cell counting is not possible).
03Obtain a pellet by centrifugation and discard the supernatant.
04Freeze the pellet at a temperature of < -18°C.
05Ship the pellet on dry ice.
A pellet prepared and shipped in this way provides suitable material for nucleic acid extraction.
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bacterial culture pelletcell pelletbacterial cell pelletbacterial culturepelletcentrifugationlogarithmic growth phasepreparation of bacteria for sequencing
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