Sample preparation is a critical stage of every study, enabling further exploration of the life sciences and investigation of genetic variation in the organism of interest. Nucleic acid samples of exceptional quality and sufficient quantity help sequencing-based projects run more efficiently and provide more reliable data for downstream bioinformatics analysis. The sample requirements listed below are the minimum volumes and concentrations required for analysis. Whenever possible, we recommend sending a larger amount of material.
Metagenome
| Service | Sample type | Amountng | Concentrationng/uL |
|---|---|---|---|
| Amplicon | gDNA | ≥500 | ≥20 |
| Metagenomics | gDNA | ≥500 | ≥20 |
| Denovo (Nanopore) | Bacteria / Fungus | gDNA | ≥2000 ≥40 |
Transcriptome
| Service | Sample type | VolumeuL | Amountug | Concentrationng/uL | RNA Integrity | Purity (NanoDrop) |
|---|---|---|---|---|---|---|
| mRNA | Total RNA | ≥10 | ≥2 | ≥50 | ≥7 | OD260/280: 1.8-2.2 OD260/230: 0.5-2.5 |
| ≥ 6 Plant | ||||||
| sRNA | Total RNA containing small RNA | ≥10 | ≥50 | ≥100 | ≥7.5 | |
| ≥7 Plant | ||||||
| lncRNA | Total RNA | ≥10 | ≥2 | ≥100 | ≥ 6.5 | |
| ≥ 6 Plant | ||||||
| circRNA | Total RNA | ≥10 | ≥2 | ≥100 | ≥ 6.5 | |
| ≥ 6 Plant | ||||||
| Nanopore | Total RNA | ≥20 | ≥2 | ≥100 | ≥8 | - |
| ≥7.5 Plant | ||||||
| PacBio | Total RNA | ≥20 | ≥2 | ≥150 | ≥9 | |
| ≥7.5 Plant |
WHOLE GENOME
| Service | Sample type | Amountug | Concentrationng/uL | Nanopore/Qubit | |
|---|---|---|---|---|---|
| Plant and animal de novo sequencing/resequencing, pangenomics and comparative genomics | PacBio CLR | gDNA | ≥10 | ≥50 | OD260/280: 1.7-2.2 OD260/230: 1.8-2.5 |
| PacBio CCS | gDNA | ≥15 | ≥300 | ||
| Nanopore | gDNA | ≥8 | ≥100 | OD260/280: 1.7-2.2 OD260/230:≥1.5 | |
| Nanopore (8K) | gDNA | ≥2 | ≥100 | ||
| NGS - Illumina | gDNA | ≥1 | ≥30 | OD260/280: 1.6-2.5 OD260/230:≥ 0.5 | |
| NGS - BGI | gDNA | ≥2 | ≥12.5 | ||
| Plant & Animal genome resequencing | PCR Product (band) | PCR | ≥3 | ≥30 | |
| PCR Product (dispersion) | PCR | ≥3 | ≥30 | ||
Pre-made libraries for sequencing
| Service | Amountng | Concentrationng/uL |
|---|---|---|
| Sequencing from premade libraries | ≥500 | ≥20 |
In exceptional cases, if the material has requirements different from those specified above (e.g. a lower concentration), these may be adjusted individually to the client's requirements following prior consultation with the Account Manager (contact@weseq.it).
For those who are unable to verify the quality of their material before shipment or who frequently have difficulty obtaining high-quality material, we additionally recommend the QC Intervention Package.
If the service includes nucleic acid extraction, please review the tissue requirements.